Gene modifiers for neuronal recovery after injury
At the Metcalfe lab, we explore innovative ways to promote axonal regeneration in adult neurons by carefully manipulating genes and proteins, while also considering critical factors like sex differences and late-onset side effects. Through meticulous experiments and precise data analysis, we ensure our discoveries are both rigorous and reproducible. With the mission of conducting translational research in meaningful ways to change people’s lives.
Why adult neurons do not regenerate?
Adult neurons, particularly in the central nervous system, typically do not regenerate due to both intrinsic and extrinsic factors. Intrinsically, mature neurons often lose their early developmental capacity for robust growth, while extrinsically, the environment surrounding an injury site contains molecules that actively inhibit regeneration, such as those found in the glial scar. Together, these factors create a hostile environment where damaged axons struggle to regrow, which underlies the enduring challenge of repairing spinal cord and brain injuries.
Our Research
In our lab, we’ve developed a cutting-edge approach that uses specialized vectors to deliver genes into adult neurons and control their activity over time. We can switch these genes on to encourage damaged nerves to regrow, then later switch them off to reduce potential side effects once healing is underway. To make this process even more precise, we use a bioluminescent “glowing” system—similar to a firefly’s light—so we can track, in real time, how effectively the neurons are responding to our treatment. This combination of targeted gene delivery and real-time monitoring gives us a powerful platform to study and eventually translate new therapies for spinal cord injury.
Where to find us
Our lab is part of the thriving neuroscience community at Kentucky Spinal Cord Injury Research Center (KSCIRC) at the University of Louisville, located on the Health Sciences Campus. We are affiliated with the Department of Anatomical Sciences and Neurobiology in the School of Medicine.